The Zhao Bioinformatics Laboratory
psRNATarget: A Plant Small RNA Target Analysis Server
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Analysis
Location:
Analysis
User-submitted small RNAs / preloaded transcripts
Preloaded small RNAs / user-submitted transcripts
User-submitted small RNAs / user-submitted transcripts
Upload small RNA sequence(s) in FASTA format:
[Load demo data]
or paste sequences below:
- file / input sequence size limit: 20M.
- invalid small RNAs will be ignored during analysis.
Select a preloaded transcript/genomic library for target search:
Allium_cepa (Onion) DFCI Gene Index (ONGI) Release 2
Arabidopsis thaliana TAIR9, cDNA, removed miRNA gene, released 06/19/2009
Arabidopsis thaliana DFCI Gene Index (AGI) Release 15
Arabidopsis thaliana, Genome(01/22/2004), 3.4K segments from +/- strand with 0.4K overlapp......
Aquilegia DFCI Gene Index (AQGI) Release 2.1
Beta_vulgaris (Beet) DFCI Gene Index (BVGI) Release 3
Brachypodium_distachyon (Purple_false_brome), JGI v1.0 genomic transcripts, ftp.jgi-psf.or......
Brachypodium_distachyon (Purple_false_brome) DFCI Gene Index (BDGI) Release 1
Brassica napus (oilseed_rape) DFCI Gene Index (BNGI) Release 3
Brassica napus (oilseed_rape) DFCI Gene Index (BNGI) Release 4
Brassica napus (oilseed_rape) DFCI Gene Index (BNGI) Release 5
Brassica rapa, de novo scaffolds assembly v1.1(2011-08-30) cds, http://brassicadb.org/brad......
Capsicum_annuum (Pepper) DFCI Gene Index (CAGI) Release 4
Carica_papaya (Papaya) DFCI Gene Index (CAPAGI) Release 1
Chlamydomonas reinhardtii DFCI Gene Index (CHRGI) Release 7
Chlorella_NC64A.best_transcripts, genome.jgi-psf.org
Chlorella_NC64A_ESTclusters,genome.jgi-psf.org
Citrus clementina (Clementine) DFCI Gene Index (CICLGI) Release 2
Citrus sinensis (Orange) DFCI Gene Index (CSGI) Release 1
Coffea canephora (coffee) SGN unigene, ftp://ftp.sgn.cornell.edu/unigene_builds/Coffea_can......
Coffeacanephora (coffee) DFCI Gene Index (COCAGI) Release 2
Ectocarpussiliculosus (Brown algae) DFCI Gene Index (BAGI) Release 1
Eucalyptus grandis, JGI genomic project, Phytozome v7.0, Egrandis_162_transcript.fa.gz
Eucalyptus grandis EST Contig, http://www.biomedcentral.com/content/supplementary/1471-216......
Euphorbia_esula (Leafy_spurge) DFCI Gene Index (EUESGI) Release 1
Festuca_arundinacea (tall_fescue) DFCI Gene Index (FAGI) Release 3
Festuca_pratensis (Meadow_ryegrass) DFCI Gene Index(MRGI) Release 1
Glycine max (soybean) DFCI Gene Index (GMGI) Release 15
Glycine max (soybean) DFCI Gene Index (GMGI) Release 16
Gossypium (cotton) DFCI Gene Index (CGI) Release 9
Gossypium (cotton) DFCI Gene Index (CGI) Release 10
Gossypium (cotton) DFCI Gene Index (CGI) Release 10.1
Gossypium (cotton) DFCI Gene Index (CGI) Release 11
Gossypium_raimondii (Cotton_raimondii) DFCI Gene Index (GORAGI) Release 1
Helianthus_annuus (Sunflower) DFCI Gene Index (HAGI) Release 6
Hordeum vulgare (barley) DFCI Gene Index (HVGI) Release 11
Ipomoea_nil (Morning_glory) DFCI Gene Index (IPNIGI) Release 1
Lactuca_sativa (Lettuce) DFCI Gene Index (LSGI) Release 3
Lactuca_serriola (Prickly_lettuce) DFCI Gene Index (LASEGI) Release 1
Linum usitatissimum (Flax/Linseed) Unigene Library, http://urgi.versailles.inra.fr/content......
Linum usitatissimum (Flax/Linseed) JGI Gene Transcripts V8.0, ftp://ftp.jgi-psf.org/pub/JG......
Lycopersicon (all tomato species) SGN mRNA sequences, as of 05-08-11, ftp://ftp.sgn.cornel......
Lycopersicon esculentum (tomato) ITAG2.3 cDNA library, http://solgenomics.net/itag/release......
Lycopersicon esculentum (tomato) ITAG2 cDNA library, http://solgenomics.net/itag/release/2......
Lycopersicon esculentum (tomato) SGN unigene, ftp://ftp.sgn.cornell.edu/unigene_builds/Tom......
Lycopersicon esculentum (tomato) DFCI Gene Index (LGI) Release 13
Lotus japonicus (lotus) DFCI Gene Index (LJGI) Release 6
Malus x domestica (apple) Genome 1.0, predicted consensus gene set CDS sequences, http://w......
Malus_x_domestica Genomic Contigs V1.0, http://www.rosaceae.org/projects/apple_genome
Malus_x_domestica (Apple) DFCI Gene Index (MDGI) Release 3
Manihot_esculenta (Cassava) DFCI Gene Index (MAESGI) Release 1
Medicago truncatula DFCI Gene index (MTGI) Release 9
Medicago truncatula DFCI Gene index (MTGI) Release 10
Medicago truncatula Mt2.0 cDNA library, http://www.medicago.org/
Medicago truncatula Mt3.0 cDNA library plus MTGI9
Mesembryanthemum_crystallinum (Ice plant) DFCI Gene Index (MCGI) Release 5
Mimulus_guttatus (Spotted_monkey_flower) DFCI Gene Index (MIGUGI) Release 1
Nicotiana benthamiana DFCI Gene Index (NBGI) Release 4
Nicotiana tabacum (tobacco) DFCI Gene Index (NTGI) Release 6
Nicotiana tabacum (tobacco) SGN unigene, ftp://ftp.sgn.cornell.edu/unigene_builds/Nicotian......
Oryza sativa (rice) TIGR genome cDNA OSA1 Release 5(OSA1R5)
Oryza sativa (rice) DFCI Gene index (OGI) Release 17
Oryza sativa (rice) DFCI Gene index (OGI) Release 18
Oryza sativa (rice) OSA1R5 Genome, 3.4K segments from +/- strand with 0.4K overlapped regi......
Oryza sativa MSU Rice Genome Annotation release 6.1 (ftp.plantbiology.msu.edu), cDNA
Panicum_virgatum (Switchgrass) DFCI Gene Index (PAVIGI) Release 1.0
Petunia_hybrida (Petunia) DFCI Gene Index (PHGI) Release 2.1
Phaseolus_coccineus (Scarlet_bean) DFCI Gene Index (PCGI) Release 1
Phaseolus_vulgaris (Bean) DFCI Gene index (PHVGI) Release 3.1
Physcomitrella patens (moss) JGI Gene Transcripts V1.1, http://genome.jgi-psf.org/Phypa1_1......
Physcomitrella_patens (Moss) DFCI Gene Index (PPSPGI) Release 1
Picea (Spruce) DFCI Gene Index (SGI) Release 4
Pinus (Pine) DFGI Gene Index (PGI) Release 8
Populus trichocarpa (poplar) JGI Gene Transcripts V1.1, http://genome.jgi-psf.org/Poptr1_1......
Populus trichocarpa (poplar) DFCI Gene Index (PPLGI) Release 5
Populus trichocarpa (poplar) Genome Assembly R1.0, 3.4K segments from +/- strand with 0.4K......
Populus trichocarpa (poplar) JGI Gene Transcripts V2.0, ftp://ftp.jgi-psf.org/pub/JGI_data......
Prunus_persica (Peach) DFCI Gene Index (PRPEGI) Release 2
Quercus (Oak) DFCI Gene Index (OGI) Release 1
Raphanus_sativus (Radish) DFCI Gene Index (RSGI) Release 1
Saccharum_officinarum (Sugarcane) DFCI Gene Index (SOGI) Release 3
Secale_cereale (Rye) DFCI Gene Index (RYEGI) Release 4
Selaginella_moellendorffii (Club_mosses) DFCI Gene Index (CMGI) Release 1
Solanum_melongena (Eggplant) DFCI Gene Index (SOMEGI) Release 1
Solanum tuberosum (potato) DFCI Gene Index (STGI) Release 13
Sorghum_bicolor (Sorghum) DFCI Gene Index (SBGI) Release 9
Striga_hermonthica (Purple_witchweed) DFCI (SHGI) Release 1
Theobroma_cacao (Cocoa) DFCI Gene Index (TCAGI) Release 3
Triphysaria DFCI Gene Index (TRIPHGI) Release 1
Triphysaria_versicolor DFCI Gene Index (TVERGI) Release 2
Triticum aestivum (wheat) DFCI Gene Index (TAGI) Release 12
Vigna_unguiculata (Cowpea) DFCI Gene Index (VUGI) Release 1
Vitis vinifera (grape) DFCI Gene Index (VVGI) Release 7
Volvox_carteri_f._nagariensis (Green_algae) DFCI Gene Index (VCGI) Release 1
Zea mays (maize) DFCI Gene Index (ZMGI) Release 19
- Request to
add / update
a transcript library.
Maximum expectation
(* Prefer lower false positive prediction rate? Please set a more stringent cut-off threshold [0-2.0]; Prefer higher prediction coverage? Please set a more relaxed cut-off threshold [4.0-5.0])
:
(range: 0-5.0)
Length for complementarity scoring (hspsize):
(range: 15-30bp)
Target accessibility - allowed maximum energy to unpair the target site (UPE):
(range: 0-100, less is better)
Flanking length around target site for target accessibility analysis
bp in upstream /
bp in downstream
Range of central mismatch leading to translational inhibition:
-
nt
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