To support the continuous development of PlantTFcat, please cite: Xinbin Dai, Senjuti Sinharoy, Michael K. Udvardi and Patrick X. Zhao, PlantTFcat: An Online Plant Transcription Factor and Transcriptional Regulator Categorization and Analysis Tool, BMC Bioinformatics, 2013, 14:321, doi:10.1186/1471-2105-14-321.
The Zhao Bioinformatics Laboratory
PlantTFcat: An Online Plant Transcription Factor and Transcriptional Regulator Categorization and Analysis Tool   
  About Analysis Family Help
Location:  Details of DICER family

  DICER
Family Type Post-transcriptional gene silencing
Plant Specific No
Functional Domains DsRNA-binding domain
Must-have InterProScan Domain Pattern IPR005034
Prohibited InterProScan Domain Pattern IPR003100
Description

The only crystal structure of full-length Dicer to date is that of G. intestinalis protein (Gi-Dicer), which consists of two endoNDs, PAZ and platform domains. The structure of Gi-Dicer has been likened to a hatchet with the dimeric endoNDs forming the blade and the PAZ at the end of the handle. Gi-Dicer cleaves dsRNA to 25 bps with 2 nt overhang at the 3? end. Using structures of bacterial RNase III-dsRNA and PAZ-nucleic acid complexes as templates, a model of Gi-Dicer - dsRNA complex was proposed. The platform domain between the endoND and PAZ appears to have a positively charged flat surface for dsRNA binding and separates the cleavage site (endoND) from the 3? end (PAZ) by ~25-bps.

Reference:

1. M. Nowotny, W. Yang, Structural and functional modules in RNA interference. Curr Opin Struct Biol 19, 286 (2009).

2. I. J. Macrae, K. Zhou, F. Li, A. Repic, A. N. Brooks, W. Z. Cande, P. D. Adams, J. A. Doudna, Structural basis for double-stranded RNA processing by Dicer. Science 311, 195 (2006).

3. I. J. MacRae, K. Zhou, J. A. Doudna, Structural determinants of RNA recognition and cleavage by Dicer. Nat Struct Mol Biol 14, 934 (2007).

Cross-reference to other databases

The family is only defined in PlantTFcat.



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