LegumeIP V3: From Models to Crops - An Integrative Gene Discovery Platform for Translational Genomics in Legumes

  • [G. max (gene)]
  • Detail of Experiment mapped on Glycine max Wm82 Gm508Wm82a4V1


NameComparative analysis of drought-responsive transcriptome in soybean lines contrasting for canopy wilting
DescriptionPurpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of abiotic stress molecular pathways. The goals of this study are to compare NGS-derived transcriptome profiling (RNA-seq) of contrasting slow wilting lines to quantify transcript abumdance under drought stress condition Overall design: Methods: The three biological replicates of DS line, Pana (control and drought samples) and DT line, PI 567690 (control and drought samples) leaf sample RNA were multiplexed and sequenced on an Illumina Hi-Seq 2000 platform. The RNA concentration of each sample was approximately 200ng/µl with a quantity of 50 µl.isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRT–PCR validation was performed using TaqMan and SYBR Green assays
ContactDigital Biology Lab, Computer Science, University of Missouri
SpeciesGlycine max
Metadata export .CSV   .JSON


Mapped Genomes Gm275Wm82a2V1     Gm508Wm82a4V1    
Expression Analysis Expression Profile /  Differential Expression /  Co-expression


Name Genotype Mutation Stage Tissue Treatment Level Time Samples Xref Download alignments
DS_control Drought Susceptible R2 stage leaf Control condition
  • DS_control_1
  • DS_control_2
  • DS_control_3
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DS_drought15d Drought Susceptible R2 stage leaf Drought stressed for 15 days
  • DS_drought15d_1
  • DS_drought15d_2
  • DS_drought15d_3
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DT_control Drought Tolerant R2 stage leaf Control condition
  • DT_control_1
  • DT_control_2
  • DT_control_3
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DT_drought15d Drought Tolerant R2 stage leaf Drought stressed for 15 days
  • DT_drought15d_1
  • DT_drought15d_2
  • DT_drought15d_3
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